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How to collect and store mosquito samples
Participants
- Public Health Entomology Research and Education Center – Eastern equine
encephalitis virus (EEEV), West Nile virus (WNV)
- Florida Medical Entomology Laboratory – St. Louis encephalitis
virus (SLEV),
WNV
- Mosquito Control Districts – SLEV, WNV, EEEV, Highlands J virus (HJV), Venezuelan
equine encephalitis virus (VEEV)
Collection techniques
- Traps: CO2-baited, CDC, New Jersey light trap, Gravid, ABC, mosquito
magnet
- Mosquitoes: Empty and gravid females only, no blood-fed
- Sample Handling:
- Samples on wet ice in field, no dry ice
- Use chill table sorts to species group in pools of 1-100
- Place female pools into cryovials
- Store at -70 C
DOH Tampa Laboratory
- Store samples at -70 C until trigger event (arbovirus in sentinel
chicken, wild bird or human in county of mosquito pool collection)
- Analysis priority:
- Culex for WNV or SLEV
- Mansonia/Coquillettidia for EEEV
- Aedes
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